IL-27 BioAssay(TM) ELISA Kit, Human

Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells.IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T(H)-17 cell development, may be a useful target for treating inflammatory diseases mediated by these cells. Intended Use: For quantitative detection of human IL-27 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Range: 156pg/ml-10,000pg/ml, Sensitivity: <10pg/ml, Specificity: No detectable crossreactivity with other cytokine. Kit Components: 143914A: Microtiter Strip 1x96wells pre-coated with anti-IL-27, Human, 143914B: Standard, Recombinant IL-27 Human, 10ng, 2x1 vial Lyophilized, 143914C: Anti-human IL-27 (Biotin), 1x130ul, USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample diluent buffer, 1x30ml, 143698: Antibody diluent buffer, 1x12ml, 143699: ABC diluent buffer, 1x12ml, 143700: TMB color developing agent, 1x10ml, 143701: TMB stop solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *143747B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add 100ul of samples and standards and incubate the plate at 37°C for 90 min. Do not wash., 2. Add 100ul biotinylated antibody and incubate the plate at 37°C for 60 min. Wash plate 3 times with 0.01M TBS or PBS., 3. Add ABC working solution and incubate the plate at 37°C for 30min. Wash plate 5 times with 0.01M TBS or PBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 20-25 min., 5. Add 100ul TMB stop solution and read.