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![IL-27 BioAssay(TM) ELISA Kit, Human](https://www.biomol.com/media/image/23/01/3a/143914-96_600x600.jpg)
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
143914.96 | 96 tests | - | - |
3 - 19 business days* |
947.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed... more
Product information "IL-27 BioAssay(TM) ELISA Kit, Human"
Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells.IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T(H)-17 cell development, may be a useful target for treating inflammatory diseases mediated by these cells. Intended Use: For quantitative detection of human IL-27 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Range: 156pg/ml-10,000pg/ml, Sensitivity: <10pg/ml, Specificity: No detectable crossreactivity with other cytokine. Kit Components: 143914A: Microtiter Strip 1x96wells pre-coated with anti-IL-27, Human, 143914B: Standard, Recombinant IL-27 Human, 10ng, 2x1 vial Lyophilized, 143914C: Anti-human IL-27 (Biotin), 1x130ul, USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample diluent buffer, 1x30ml, 143698: Antibody diluent buffer, 1x12ml, 143699: ABC diluent buffer, 1x12ml, 143700: TMB color developing agent, 1x10ml, 143701: TMB stop solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *143747B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add 100ul of samples and standards and incubate the plate at 37°C for 90 min. Do not wash., 2. Add 100ul biotinylated antibody and incubate the plate at 37°C for 60 min. Wash plate 3 times with 0.01M TBS or PBS., 3. Add ABC working solution and incubate the plate at 37°C for 30min. Wash plate 5 times with 0.01M TBS or PBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 20-25 min., 5. Add 100ul TMB stop solution and read.
Keywords: | p28, IL27, IL27A, IL27-A, IL-27-A, Interleukin-30, IL-27 subunit alpha, Interleukin-27 subunit alpha |
Supplier: | United States Biological |
Supplier-Nr: | 143914 |
Properties
Application: | ELISA |
Species reactivity: | human |
Format: | Solid Phase |
Database Information
KEGG ID : | K22629 | Matching products |
UniProt ID : | Q8NEV9 | Matching products |
Gene ID | GeneID 246778 | Matching products |
Handling & Safety
Storage: | vT |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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