IL-12(p70) BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human IL-12(p70) in cell culture supernates and serum. Interleukin (IL)-12 is a 70kD cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response.[i] And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.[ii] Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.[iii] The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75kD. , Assay Range:, 7.8pg/ml-500pg/ml, Sensitivity: < 2 pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: O60595, Entrez Gene ID: 3592, Kit Components: 96-well plate pre-coated with anti-IL-12(p70), Human, Lyophilized recombinant IL-12(p70), Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.