Growth Hormone Releasing Hormone (GHRH) BioAssay(TM) ELISA Kit (Human)

Growth Hormone Releasing Hormone (GHRH) BioAssay(TM) ELISA Kit (Human) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GHRH in human serum, plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants and Other Biological Fluids. Detection Range: 12.35-1000pg/ml, Sensitivity: ?6.15pg/ml, Intra-Assay: CV <10% , Inter-Assay: CV <12%, Kit Components: *025511A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use, *025511B: Standard, 2x1vial , 025511C: Standard Diluent, 1x20ml, *025511D: Detection Reagent A, 1x120ul , *025511E: Detection Reagent B, 1x120ul , 025511F: Assay Diluent A, 2x, 1x6ml, 025511G: Assay Diluent B, 2x, 1x6ml, 025511H: TMB Substrate, 1x9ml, 025511K: Stop Solution, 1x6ml, 025511L: Wash Buffer, 30X, 1x20ml, Precaution: The Stop Solution (025511K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *025511A, *025511B, *025511D and *025511E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 50ul standard or sample to each well, then add 50ul working Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C., 3. Aspirate and wash 3 times., 4. Add 100ul working Detection Reagent B. Incubate 30 minutes at 37°C., 5. Aspirate and wash 5 times., 6. Add 90ul 025511H: Substrate Solution. Incubate 15-25 minutes at 37°C., 7. Add 50ul 025511K: Stop Solution. Read at 450nm immediately.