Bispecific Claudin-18.2:CD3 Bridging Chemiluminescence ELISA Kit

The Bispecific Claudin-18.2:CD3 Bridging Chemiluminescence ELISA Kit is an ELISA designed to analyze the ability of Bispecific Antibodies (BsAbs) to bridge Claudin-18.2 (Claudin-18, Isoform 2) and CD3 (cluster of differentiation 3) for screening and profiling applications. This assay kit can determine if an anti-Claudin-18.2-anti-CD3 bispecific antibody binds to both targets simultaneously and bridges Claudin-18.2 to CD3. The Bispecific Claudin-18.2:CD3 Bridging Chemiluminescence ELISA Assay Kit comes with enough recombinant human Claudin-18.2, CD3- Containing Detection Reagent, and assay buffer for 100 enzyme reactions. This kit also includes Anti-Claudin-18.2-Anti-CD3 Bispecific Antibody as a positive control. A 96-well plate is coated with Claudin-18.2 protein. After coating, an anti-Claudin-18.2-anti-CD3 bispecific antibody is added in an optimized assay buffer. The plate is washed to remove the unbound antibody. The plate is then incubated with CD3-Containing Detection Reagent. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the bridging efficacy. Need us to run inhibitor screens or profile your bispecific antibodies towards CD3 and claudin-18.2? Check out our Immunotherapy Biochemical Screening Services.