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![Arachidonate-12-Lipoxygenase (ALOX12) BioAssay(TM) ELISA Kit (Human)](/custom/plugins/NetiThemeBiomol/Resources/Themes/Frontend/Biomol/frontend/_public/src/img/no-picture.jpg)
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
152301.96 | 96 tests | - | - |
3 - 19 business days* |
970.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Arachidonate-12-Lipoxygenase (ALOX12) BioAssay(TM) ELISA Kit (Human) is a Sandwich enzyme... more
Product information "Arachidonate-12-Lipoxygenase (ALOX12) BioAssay(TM) ELISA Kit (Human)"
Arachidonate-12-Lipoxygenase (ALOX12) BioAssay(TM) ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase (ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Arachidonate-12-Lipoxygenase (ALOX12) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: 0.313-20ng/ml, Sensitivity: <0.116ng/ml, Sample Matrix: Tissue homogenates, cell lysates and other biological fluids. Cross Reactivity: This assay has high sensitivity and excellent specificity for detection of Arachidonate-12-Lipoxygenase (ALOX12). No significant cross-reactivity or interference between Arachidonate-12-Lipoxygenase (ALOX12) and analogues was observed. Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Storage and Stability: Desiccation recommended. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt. Store all other reagents at 4°C. Stable for 6 months after receipt of order.
Keywords: | 12LO, ALOX12, 12S-LOX, EC=3.3.2.-, EC=1.13.11.31, 12S-lipoxygenase, Lipoxin synthase 12-LO, Platelet-type lipoxygenase 12, Arachidonate 12-lipoxygenase, 12S-type |
Supplier: | United States Biological |
Supplier-Nr: | 152301 |
Properties
Application: | ELISA |
Species reactivity: | human |
Format: | Solid Phase |
Database Information
KEGG ID : | K00458 | Matching products |
UniProt ID : | P18054 | Matching products |
Gene ID | GeneID 239 | Matching products |
Handling & Safety
Storage: | vT |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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