Angiostatin K1-3 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Angiostatin K1-3 in cell culture supernates, serum and plasma. Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED?? =70 nM) relative to kringles 1-4 (ED?? = 135 nM). , Assay Range:, 156pg/ml-10,000pg/ml, Sensitivity: < 10 pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P00747, Entrez Gene ID: 5340, Kit Components: 96-well plate pre-coated with anti-Angiostatin K1-3, Human, Lyophilized recombinant Angiostatin K1-3, Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.