Cell Meter(TM) Fluorimetric Phagocytosis Assay Kit *Green Fluorescence*

Product key features: Utilizes unique Protonex(TM) Green 500-Latex Beads for enhanced detection Exhibits minimal background due to the fluorogenic properties of Protonex(TM) Green 500-Latex Beads Convenient Ex/Em wavelength well matches the standard FITC filter set Includes a red fluorescent cell viability dye for accurate live cell detection Suitable for various applications including microscopy, microplate assays, and flow cytometry. Product description: Cell Meter(TM) Fluorimetric Phagocytosis Assay Kit uses the unique Protonex(TM) Green 500-based latex bead conjugate. Protonex(TM) Green 500 bead conjugate is a unique pH probe that can be delivered into cells by phagocytosis. The bead conjugate is a ready to use suspension. Unlike most of the existing fluorescent dyes, the Protonex(TM) Green 500-latex bead conjugate is non-fluorescent. However, its fluorescence dramatically increases as the bead conjugate gets into acidic phagosomes/phagolysosomes. The spectral properties of Protonex(TM) Green 500 are similar to those of FITC, allowing for the use of common FITC filter sets in assays that use Protonex(TM) Green 500-latex bead conjugates. It provides a robust tool to study phagocytosis and its regulations. Cell Meter(TM) Fluorimetric Phagocytosis Assay Kit also includes a red fluorescent cell viability dye, which allows the simultaneous detection of both live cells and the process of phagocytosis by fluorescent microscopy. This assay can be adapted for either fluorescence microplate reader or flow cytometry detection. Phagocytosis is the cellular uptake of particles within a plasma-membrane envelope by a cell. The process is critical in the innate immune response by engulfment and destruction of invading microorganisms. Phagocytosis is required in maintaining tissue homeostasis and remodeling by the clearance of apoptotic bodies. Once internalized, the phagosome fuses with lysosomes to form a secondary phagolysosome for digestion, resulting in progressive decrease of pH.