Cell Meter(TM) Fluorimetric Intracellular Total ROS Activity Assay Kit*Orange Fluorescence*

Ex: 556 nm. Em: 566 nm. Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways. Cell MeterT Fluorimetric Intracellular Total ROS Activity Assay Kit uses our proprietary ROS BriteT 570 sensor to quantify ROS in live cells. The cell-permeable and non-fluorescent ROS BriteT 570 exhibits a strong fluorescence signal upon reaction with ROS. ROS BriteT 570 sensor is localized in the cytoplasm. The fluorescence signal of ROS BriteT 570 sensor can be measured by fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry. The Cell MeterT Fluorimetric Intracellular Total ROS Activity Assay Kit provides a sensitive, one-step fluorimetric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format using either a fluorescence microplate reader at Ex/Em = 540/570 nm or a fluorescent microscope with TRITC filter.