Cell Meter(TM) Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Deep Red Fluorescence*

Cell Meter(TM) TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation. The assay is non-radioactive and rapid. The TUNEL assay uses the terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of TF5-dUTP at the free 3'-hydroxyl ends of the fragmented DNAs. The resulted TF5-labeled DNAs are analyzed by fluorescence microscopy (Cy5 filter set) or flow cytometry with 633 or 640 nm laser with 660/20 nm filter (APC channel). Its red emission can be conveniently multiplexed with GFP labelled targets. Direct incorporation of fluorescent TF5-labeled nucleotides significantly reduces the number of test steps. The kit is optimized to detect apoptosis in fixed cells and formalin-fixed, paraffin-embedded tissue sections.