Cell Meter(TM) Caspase 3/7 Activity Apoptosis Assay Kit (Red Fluorescence)

Our Cell Meter(TM) assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring Caspase 3 activation. Caspase 3 is widely accepted as a reliable indicator for cell apoptosis since the activation of caspase-3 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses Z-DEVD-ProRed(TM) as the fluorogenic indicator for caspase-3 activity. Cleavage of ProRed(TM) DEVD blocking peptide residue by caspase 3 generates strongly red fluorescent ProRed(TM) that is monitored fluorimetrically at about 620 nm with excitation of about 530 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays. Using 100 ul of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 100 assays. Using 25 ul of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 400 assays.