Cell-based Assays

In modern life science and pharmaceutical research cell-based assays are becoming increasingly important. Real-time measurements with cells offer the possibility of successful quantification of cell viability, cytotoxicity and biological activity. By realistically representing the situation in vivo, these assays enable direct analysis of cell responses and play an important role in understanding disease mechanisms.

In modern life science and pharmaceutical research cell-based assays are becoming increasingly important. Real-time measurements with cells offer the possibility of successful quantification of... read more »
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Cell-based Assays

In modern life science and pharmaceutical research cell-based assays are becoming increasingly important. Real-time measurements with cells offer the possibility of successful quantification of cell viability, cytotoxicity and biological activity. By realistically representing the situation in vivo, these assays enable direct analysis of cell responses and play an important role in understanding disease mechanisms.

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Magic Red(TM) Cathepsin B Assay Kit
Magic Red(TM) Cathepsin B Assay Kit

Item number: ICT-937

This kit is based on a cell-permeant fluorescent probe (MR-(RR)2) designed for real-time detection of cathepsin B activity within whole living cells, using a fluorescence microscope. Excitation wavelength 560nm, emission 610-650nm.
Application: Real time FM, FC
305.00€ *
Review
One-Step Luciferase Assay System
One-Step Luciferase Assay System

Item number: BPS-60690-1

The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component...
Keywords: Luciferin, Firefly luciferase, luciferase buffer,
Application: Firefly luciferase assays, HTS
From 265.00€ *
Review
Cell Meter(TM) Caspase 3/7 Activity Apoptosis Assay Kit (Red Fluorescence)
Cell Meter(TM) Caspase 3/7 Activity Apoptosis Assay Kit...

Item number: ABD-22797

Our Cell Meter(TM) assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring Caspase 3 activation. Caspase 3 is widely accepted as a reliable indicator for...
Keywords: CASP3, CPP32, SCA-1, CPP-32, CASP-3, Apopain, Caspase-3, EC=3.4.22.56, MCH3, CASP7, CMH-1, CASP-7, ICE-LAP3, Caspase-7,...
Application: Caspase-3 assays
426.00€ *
Review
AR (human) Reporter Assay Kit
AR (human) Reporter Assay Kit

Item number: IB-IB03001

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of full-length, unmodified human androgen receptor (NR3C4), a ligand-dependent transcription factor commonly referred to as AR. INDIGO's reporter cells include the luciferase...
Keywords: AR, DHTR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
Application: Functional activity screening
Species reactivity: human
From 1,507.00€ *
Review
Cell Meter(TM) TUNEL Apoptosis Assay Kit
Cell Meter(TM) TUNEL Apoptosis Assay Kit

Item number: ABD-22844

DNA fragmentation represents a characteristic of late stage apoptosis. DNA fragmentation in apoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL). The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that...
Application: TUNEL apoptosis assay
426.00€ *
Review
Oxygen Consumption Rate Assay Kit
Oxygen Consumption Rate Assay Kit

Item number: Cay600800-96

The oxygen consumption rate (OCR) of cells is an important indicator of normal cellular function. It is used as a parameter to study mitochondrial function as well as a marker of factors triggering the switch from healthy oxidative phosphorylation to aerobic glycolysis in cancer cells. Oxygen consumption is...
Keywords: OCR
Application: Fluorescence
554.00€ *
Review
RORgamma (human) Reporter Assay Kit
RORgamma (human) Reporter Assay Kit

Item number: IB-IB04001

This human RAR-related orphan receptor gamma (RORgamma) mRNA is expressed from the RORC gene in two forms via alternate usage of tissue-specific promoters. Variant 1 mRNA is expressed in numerous tissues, and encodes receptor isoform 1, referred to as RORgamma. Variant 2 mRNA comprises an alternate exon 1 that...
Keywords: RORC, NR1F3, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, RAR-related orphan receptor C, Retinoid-related...
Application: Functional activity screening
Species reactivity: human
From 1,507.00€ *
Review
Human GHR Reporter Assay System
Human GHR Reporter Assay System

Item number: IB-IB14001

The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist. This kit product is an all-inclusive assay system that includes, in addition to Reporter Cells, two optimized media for use during cell culture and (optionally) in...
Keywords: GHR
Application: Functional activity screening
Species reactivity: Human
From 1,507.00€ *
Review
FLICA(TM) 660 Caspase 3/7 Assay Kit, far-red fluorescence  (25-50 tests)
FLICA(TM) 660 Caspase 3/7 Assay Kit, far-red fluorescence...

Item number: ICT-9125

The far-red FLICA® 660-DEVD-FMK caspase-3 detection probe is comprised of the preferred affinity peptide sequence (DEVD) targeted by activated caspase-3 and caspase-7, a far-red fluorescent 660 dye label, and a fluoromethyl ketone (FMK) reactive moiety. The resulting fluorescent caspase-3 inhibitor probe forms an...
Keywords: Apoptosis kit, CASP3, CPP32, SCA-1, CPP-32, CASP-3, Apopain, Caspase-3, EC=3.4.22.56, Protein Yama, Cysteine protease...
Application: FM, FC, Apoptosis detection
379.00€ *
Review
FAM-FLICA(R) Caspases 3 & 7 Assay Kit
FAM-FLICA(R) Caspases 3 & 7 Assay Kit

Item number: ICT-93

This kits makes use of a cell-permeant green fluorecent probe (FAM-DEVD-FMK ) that binds to active caspase-3 & 7 in living cells undergoing apoptosis. Excitation wavelength 488-492nm, emission 515-535nm.
Keywords: Apoptosis kit
Application: FM, FC, Apoptosis detection
320.00€ *
Review
Cell Meter(TM) JC-10 Mitochondrial Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays
Cell Meter(TM) JC-10 Mitochondrial Membrane Potential...

Item number: ABD-22801

Although JC-1 is widely used in many labs, its poor water solubility causes extraordinary inconvenience. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 is developed to be a superior alternative to JC-1 where high dye concentration is desired. Compared to JC-1, JC-10 has much better...
Application: FC, Apoptosis studies
426.00€ *
Review
PDGFRa Reporter Assay System (human)
PDGFRa Reporter Assay System (human)

Item number: IB-IB23101

This is an all-inclusive cell-based luciferase reporter assay kit targeting the Human Platelet-Derived Growth Factor Receptor alpha (PDGFRalpha). INDIGO's PDGFRalpha reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the Human Platelet-Derived Growth...
Keywords: CD140a, PDGFR2, PDGFRA, PDGFR-2, PDGFR-alpha, EC=2.7.10.1, PDGF-R-alpha, CD140a antigen, CD140 antigen-like family member...
Application: Functional activity screening
Species reactivity: human
From 1,507.00€ *
Review
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