Xanthine Oxidase (XOD) Activity Fluorometric Assay Kit

Detection principle Hypoxanthine are oxidized by xanthine oxidase (XOD) to produce xanthine and super oxygen anion, which will quickly converte to hydrogen peroxide in the system, and then, in the role of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe to fluorescent substance. By measuring the fluorescence value, the corresponding the activity of xanthine oxidase can be calculated. Performance characteristics Synonyms XOD Sample type Serum, plasma, animal tissue Sensitivity 0.01 U/L Detection range 0.01 -1.2 U/L Detection method Fluorescence method Assay type Enzyme Activity Assay time 25 min Precision Average inter-assay CV: 9%Average intra-assay CV: 4.1% Other instruments required Vortex mixer, Centrifuge Storage -20 °C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01 -1.2 U/L). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor Mouse serum 1 Dog serum 3-5 Rat plasma 1 10% Rat kidney tissue homogenate 5-10 Horse serum 1 Human plasma 1 10% Mouse heart tissue homogenate 5-10 10% Rat lung tissue homogenate 5-10 Note: The diluent is reagent 1.