Uric Acid Assay Kit

Cayman's Uric Acid Assay Kit provides fluorescence- or colorimetric-based methods for detecting uric acid in plasma, serum, and urine. In the assay, uricase catalyzes the conversion of uric acid to allantoin, hydrogen peroxide (H2O2), and carbon dioxide. H2O2, in the presence of horseradish peroxidase, reacts stoichiometrically with ADHP (10-acetyl-3,7,-dihydroxyphenoxazine) to produce the highly fluorescent compound resorufin. Resorufin fluorescence can be analyzed with an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm. Alternatively, the absorbance of resorufin can be measured at 570 nm.Synonyms: Urate Assay Kit. Emission: 585-595 nm (fluorometric). Excitation: 530-540 nm (fluorometric). Limit of Detection: 0.18 µM (fluorometric), 0.45 µM (colorimetric). Assay Range: 5-100 µM (fluorometric), 5-1,000 µM (colorimetric). Lower Limit of Quantification: 5 µM (fluorometric), 50 µM (colorimetric). Origin: Plant/Armoracia rusticana.