Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)

Detection principle Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to produce quinones which is proportional to the content of TG. Performance characteristics Synonyms: TG Sample type: serum, plasma, cells and tissue Sensitivity: 0.14 mmol/L Detection range: 0.14-10 mmol/L Detection method: Colorimetric method Assay type: Quantitative Assay time: 30 min Precision: Average inter-assay CV: 9.2%Average intra-assay CV: 4.1% Other instruments required: Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge Other reagents required: Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol(AR) Storage: 2-8? Valid period: 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.14-10 mmol/L). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor Human serum 1 Mouse serum 1 Rat plasma 1 10% Mouse liver tissue homogenate 1 10% Mouse kidney tissue homogenate 1 10% Mouse heart tissue homogenate 1 HepG2 cells 1 Note: The diluent of serum (plasma) is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), The diluent of animal tissue and cells is isopropanol.