Screen Quest(TM) Fluorimetric Neutrophil Elastase Inhibitor Screening Kit

The Screen Quest(TM) Fluorimetric Neutrophil Elastase Inhibitor Screening Kit offers a fast, sensitive, and high-throughput method for screening and characterizing potential inhibitors of NE. This assay measures the NE activity based on hydrolysis of the substrate, producing a fluorescent product with Ex/Em=360/470nm. The amount of fluorescent product is directly proportional to the enzymatic activity of the neutrophil elastase present in the sample. Neutrophil elastase, also known as leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil, elaszym, serine elastase, subtype human leukocyte elastase (HLE) is a cytotoxic Serine Protease with a broad substrate specificity. Azurophil granules of neutrophil store NE and release them in response to multiple stimuli like pathogens, immune complexes, or chemotactic agents such as PMA causing degradation of a range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. NE hydrolysis accounts for approximately 80% of the total protease hydrolysis activity in the human body. Disease implications of NE include cystic fibrosis, COPD, lung emphysema, rheumatoid arthritis, and adult respiratory distress syndrome. NE has also been associated with lung injury seen in COVID-19.