Phenylalnine Ammonia Lyase (PAL) Activity Assay Kit

Detection principle Phenylalanine ammonia lyase (PAL) can catalyze L-phenylalanine to produce trans-cinnamic acid and ammonia, and trans-cinnamic acid has the maximum absorption peak at 290 nm. PAL activity can be calculated by measuring the increase of OD value at 290 nm. Performance characteristics Synonyms PAL Sample type Serum, plasma, animal tissue, cells Sensitivity 0.78 U/g tissue Detection range 0.78-156 U/g tissue Detection method Colorimetric method Assay type Enzyme Activity Assay time 70 min Precision Average inter-assay CV: 4.6%Average intra-assay CV: 3.1% Other instruments required Tubes, Micropipettor, Vortex mixer, 37°C Incubator Storage 2-8 °C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.78-156 U/g tissue). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor 10% Epipremnum aureum tissue homogenate 1 10% Carrot tissue homogenate 1 10% Green pepper tissue homogenate 1 10% Corn grain tissue homogenate 1 Note: The diluent is reagent 1.