PARP1 assay kit

The PARP1 Chemiluminescent Activity Assay kit is designed to measure PARP1 activity using purified PARP1 or PARP1 extracts. PARP1 is known to catalyze the NAD-dependent addition of poly, ADP-ribose, to histones. The PARP1 assay kit comes in a convenient 96-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 100 enzyme reactions. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with the assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.