MLL1 (KMT2A): WDR5 Binding Chemiluminescent Assay Kit

The MLL1 (KMT2A): WDR5 Binding Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the binding between MLL1 (mixed lineage leukemia protein-1, also known as KMT2A) and WDR5 (WD40 repeat-containing protein 5) for screening and profiling applications. The MLL1 (KMT2A): WDR5 Binding Chemiluminescent Assay Kit comes with enough purified MLL1 (amino acids 3745-end) and WDR5 proteins, primary and secondary antibody, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with MLL1 protein. After coating and blocking, WDR5 is added in an optimized assay buffer. Unbound WDR5 is washed away, and the plate is incubated with a primary antibody followed by a secondary HRP-conjugated antibody. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of WDR5 binding to MLL1 (KMT2A).