Maltase Activity Assay Kit

Detection principle Maltase catalyze the corresponding substrate to produce monosaccharide. Monosaccharide produce hydrogen peroxide under the action of oxidase. Hydrogen peroxide react with chromogenic agent to form red product. The activity of maltase can be calculated by detection the optical density with spectrophotometer at 505 nm. Performance characteristics Sample type Animal tissue Sensitivity 6.32 U/mL Detection range 6.32-750 U/mL Detection method Colorimetric method Assay type Enzyme Activity Assay time 60 min Precision Average inter-assay CV: 5.7%Average intra-assay CV: 2.7% Other instruments required Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) Storage 2-8 °C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (6.32-750 U/mL). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor 10% Rat heart tissue homogenate 1 10% Rat liver tissue homogenate 1 10% Rat spleen tissue homogenate 1 10% Mouse intestinal tissue homogenate 1 10% Mouse kidney tissue homogenate 1 10% Mouse brain tissue homogenate 1 Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).