Lactate dehydrogenase (LDH) Activity Assay Kit

Detection principle Lactate dehydrogenase (LDH) is an oxidoreductase. LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. LDH is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein. LDH is released into the blood by cells after tissue damage or erythrocyte hemolysis. Extracellular LDH activity is used to detect cell damage or cell death. Performance characteristics Synonyms LDH Sample type Serum, plasma, tissue, cells Sensitivity 4 U/L Detection range 4-400 U/L Detection method Colorimetric method Assay type Enzyme Activity Assay time 70 min Precision Average inter-assay CV: 7.2%Average intra-assay CV: 2.2% Other instruments required Micropipettor, Vortex mixer, Water bath Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) Storage 2-8 °C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (4-400 U/L). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor Human serum 10-15 Human plasma 10-15 Porcine serum 20-30 Rat serum 20-30 Rat plasma 20-30 10% Rat liver tissue homogenate 800-1000 10% Mouse kidney tissue homogenate 500-800 10% Rat lung tissue homogenate 300-500 Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).