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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
BPS-78570 | 384 rxns | - | - |
3 - 15 business days* |
1,965.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The KRAS(G12D) Coupled Nucleotide Exchange Assay Kit is designed for screening and profiling of... more
Product information "KRAS(G12D) Coupled Nucleotide Exchange Assay Kit"
The KRAS(G12D) Coupled Nucleotide Exchange Assay Kit is designed for screening and profiling of KRAS(G12D) antagonists/inhibitors by monitoring the binding of an effector protein such as the Ras binding domain of Raf1, (RBD-cRaf) to KRAS(G12D). With this kit, a few simple steps on a microtiter plate are required for nucleotide exchange detection. First, a sample containing GDP-loaded KRAS(G12D) is incubated with SOS1 and GTP for the nucleotide exchange. Next, RBD-cRAF is added and incubated for the effector-RAS binding. Then, acceptor and donor beads are added and incubated for detection followed by reading the Alpha-counts. SOS1 (son of sevenless) is a guanine nucleotide exchange factor that facilitates the exchange of GDP for GTP. GDP-loaded KRAS(G12D) is in an inactive state and does not interact with the Ras-binding domain (RBD) of cRAF. SOS1 assists in the release of GDP from KRAS(G12D) so that GTP can occupy the nucleotide binding pocket. This results in a conformational change in KRAS(G12D) that permits its binding to RBD-cRAF. The KRAS(G12D) Coupled Nucleotide Exchange Assay Kit utilizes GST-tagged RBD-cRAF and His-tagged KRAS (G12D) to assay binding of KRAS(G12D) to RBD-cRAF in the Alpha assay. Glutathione acceptor and Ni chelate donor beads are brought into proximal range by the binding of KRAS(G12D) and RBD-cRAF, enabling the energy transfer from the donor to acceptor beads after laser excitation.
Keywords: | KRAS, KRAS2, |
Supplier: | BPS Bioscience |
Supplier-Nr: | 78570 |
Properties
Application: | Small molecule inhibitor/antagonist screening, HTS |
Species reactivity: | human |
Database Information
KEGG ID : | K07827 | Matching products |
UniProt ID : | P01116 | Matching products |
Gene ID | GeneID 3845 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | -20°C (International: °C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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