Fluorogenic SIRT1 (Sir2) Assay Kit

The Fluorogenic SIRT1 Assay Kit is a complete assay system designed to measure Sirtuin 1 (SIRT1) activity for screening and profiling Applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT1 (Sir2) activity measurements. In addition, the kit includes purified SIRT1 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT1 activity level. First, the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated lysine side chain, is incubated with a sample containing SIRT1 activity (e.g. HeLa nuclear extract or purified SIRT1 enzyme). The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader. Components: SIRT1 human recombinant enzyme, 50 µg, Fluorogenic SIRT substrate 1, 50 µl at 5 mM, Nicotinamide Adenine Dinucleotide (NAD), 50 µl at 50 mM, Nicotinamide, 500 µl at 10 mM, 2x SIRT Developer (contains 2 mM Nicotinamide), 6 ml, SIRT assay buffer, 10 ml, 1 black, low binding NUNC black microtiter plate. Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.