Fluorogenic HDAC8 Assay Kit

The Fluorogenic HDAC8 Assay Kit is a complete assay system designed to measure histone deacetylases 8 (HDAC8) activity in cell extracts, nuclear extracts, immunoprecipitates, and purified enzymes. It comes in a convenient 96-well format, with all the reagents necessary for fluorescent HDAC activity measurements. In addition, the kit includes purified HDAC8 and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC8 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC8 activity level. First, the HDAC fluorometric substrate is incubated with a sample containing HDAC activity (e.g. HeLa nuclear extract or purified HDAC8 enzyme). The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.