cGAS Inhibitor Screening Assay Kit

Cayman's cGAS Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human cGAS, which is a cytosolic dsDNA sensor that detects microbial pathogens. The assay directly measures 2'3'-cGAMP produced by cGAS in the presence of DNA, ATP, and GTP. The cyclic dinucleotide product of that reaction is quantified via ELISA using a 2'3'-cGAMP-specific antiserum. The cGAS inhibitor CU-76 is included as a positive control. The ELISA portion of Cayman's cGAS Inhibitor Screening Assay Kit is a competitive assay with a range of 4.57-10,000 pM, a midpoint of approximately 300 pM (50% B/B0), and a sensitivity (80% B/B0) of approximately 50 pM.Synonyms: C6orf150, 2'3'-cGAMP Synthase, Cyclic GMP-AMP Synthase. Limit of Detection: 5.0 pM. Assay Range: 4.6-10,000 pM. Sensitivity: 48.4 pM. Mid-point: 300.1 pM. License: This product was developed in conjunction with BIOLOG Life Science Institute.. Origin: Animal/Bovine, Animal/Rabbit.