Cell Ferrous Iron Colorimetric Assay Kit

Detection instrument: Microplate reader. This kit can measure errous ions (Fe2+) content in cell sample. Detection principle: Iron is one of the metal elements in organism and has important physiological functions. Ferrous ion is a key element in heme and hemoglobin and plays an important role in many biochemical reactions. Ferrous ions (Fe2+) in samples can bind with probe to form complexes, which has a maximum absorption peak at 593 nm. The concentration of ferrous ions can be calculated by measuring the OD value at 593 nm indirectly. Sample type: Cells. Research area: Inorganic ions. Assay time: 40 min. Sensitivity: 0.4 µmol/L. Detection range: 0.4-50 µmol/L. Recovery rate: 99. Inter CV: 1.5. Intra CV: 1.3