Total Protein (TP) Colorimetric Assay Kit (Biuret Method)

Detection principle Any compound that contains two -CONH2 in the molecule can react with alkaline copper solution to form a purple complex, which is known as the biuret reaction. Many peptide bonds (-CONH-) in protein molecules can perform this reaction, and the color degree of all kinds of proteins are essentially the same. Performance characteristics Synonyms TP Sample type Serum,plasma,tissue Sensitivity 0.373 g/L Detection range 0.373-80 g/L Detection method Colorimetric method Assay type Quantitative Assay time 50 min Precision Average inter-assay CV: 2.6%Average intra-assay CV: 1.2% Other instruments required Micropipettor, Incubator Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) Storage Reagent 3: -20 °C, others: 2-8 °C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.373-80 g/L). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor Porcine serum 2-4 Human serum 2-4 5% Mouse liver tissue homogenization 1 10% Epipremnum aureum tissue homogenization 1 5% Mouse heart tissue homogenization 1 Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).