Amplite(TM) Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*

Ex: 571 nm. Em: 585 nm. Most of commercial protein kinase assay kits are either based on monitoring of phosphopeptide formation or ATP deletion. For the kinase assay kits that are based on detection of phosphopeptides one has to spend time and efforts to identify an optimized peptide substrate while the ATP depletion method suffers various interferences due to the use of luciferase that are inhibited or activated by various biological compounds. The Amplite(TM) Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities.