Acetylcholinesterase (AchE) Activity Assay Kit

Detection principle AchE catalyzes the hydrolysis of acetylcholine to form choline, and choline react with dithio p-nitrobenzoic acid (DTNB) to form 5-mercapto-nitrobenzoic acid (TNB). TNB has an absorption peak at 412nm. And the activity of AchE is calculated by measuring the increasing rate of absorbance at 412nm. Performance characteristics Synonyms AchE Sample type Serum,plasma,animal tissue Sensitivity 1.225 U/mL Detection range 1.225-490 U/mL Detection method Colorimetric method Assay type Enzyme Activity Assay time 20 min Precision Average inter-assay CV: 9.300000000000001%Average intra-assay CV: 4.7% Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) Storage 2-8 °C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (13.88-320.13 U/mL). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor Mouse serum 8-20 Mouse plasma 4-10 Human serum 4-10 Human plasma 4-10 Rat serum 4-10 Dog serum 4-10 Horse serum 2-8 10% Mouse liver tissue homogenate 1 10% Mouse kidney tissue homogenate 1 10% Mouse brain tissue homogenate 2-8 10% Crucian carp muscle tissue homogenate 1 Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).