Fluorescent TrueBlot(R): Anti-Rabbit IgG DyLight(TM), clone eB182, DyLight(TM) 800 Conjugated

Fluorescent Rabbit TrueBlot(R) Antibody DyLight(TM) 800 Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity is observed against native Rabbit IgG by both Western blot and ELISA. Rabbit IgG TrueBlot(R) is a unique DyLight(TM) 800 conjugated anti-rabbit IgG immunoblotting (second step) reagent. Rabbit IgG TrueBlot(R) enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Fluorescent Western Blot data with Rabbit IgG TrueBlot(R), simply substitute the conventional DL800 anti-rabbit IgG blotting reagent with Fluorescent Rabbit TrueBlot(R) Antibody DyLight(TM) 800 and follow the prescribed protocol for sample preparation and immunoblotting. Rabbit IgG TrueBlot(R) is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot(R) with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Fluorescent Rabbit TrueBlot(R) Antibody DyLight(TM) 800 may also be used for detection in immunoassays that do not employ immunoprecipitation.