Anti-phospho-Protein Kinase A, alpha/beta, Catalytic Subunit (Thr197) (PKAa,b)

c-AMP-dependent Protein Kinase (PKA) is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits that dissociate upon activation by cAMP. The catalytic subunit of PKA contains the activation loop and mediates DNA binding and substrate recognition. The catalytic subunit is assembled and expressed as an active form and is phosphorylated on threonine 197 by PDK-1 in the activation loop. Phosphorylation of threonine 197 is critical for PKA maturation, optimal conformation and catalytic activity and plays a key role in mediating its biological functions. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 0.1-0.75ug/ml. At 0.35ug/ml, the dilution provides 100ml working solution, which at 10ml/blot allows 10 blots to be performed. , Optimal dilutions to be determined by the researcher. Positive Control: Forskolin-treated NIH3T3 cells, or Y-1 mouse adrenal cortical cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.