Anti-phospho-Catenin, beta (Ser675)

B-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates B-catenin on Ser45. This phosphorylation event primes B-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3B destabilizes B-catenin by phosphorylating it at Ser33, Ser37 and Thr41 (7). Mutations in these phosphorylation sites, which result in the stabilization of B-catenin protein levels, have been found in many tumor cell lines (8). PKA was shown to phosphorylate B-catenin at Ser675. Phosphorylation at Ser675 induces B-catenin accumulation in the nucleus and increases its transcriptional activity (9,10). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunoprecipitation: 1:200, Immunofluorescence (IF-IC): 1:100, Immunofluorescence (frozen): 1:100., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.