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![Anti-Histone H2B, acetylated (Lys12) (Histone H2B Type 1-B, Histone H2B.f, H2B/f, H2BFF, Histone H2B](/custom/plugins/NetiThemeBiomol/Resources/Themes/Frontend/Biomol/frontend/_public/src/img/no-picture.jpg)
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
H5110-09Z.100 | 100 µl | - | - |
3 - 19 business days* |
757.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary... more
Product information "Anti-Histone H2B, acetylated (Lys12) (Histone H2B Type 1-B, Histone H2B.f, H2B/f, H2BFF, Histone H2B"
The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination. Histone acetylation occurs mainly on the amino-terminal tail domains of histones H2A (Lys5), H2B (Lys5, 12, 15, and 20), H3 (Lys9, 14, 18, 23, 27, and 56), and H4 (Lys5, 8, 12, and 16), and is important for the regulation of histone deposition, transcriptional activation, DNA replication, recombination and DNA repair. Hyper-acetylation of the histone tails neutralizes the positive charge of these domains and is believed to weaken histone-DNA and nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and increasing the access of DNA to various DNA-binding proteins. In addition, acetylation of specific lysine residues creates docking sites for a protein module called the bromodomain, which binds to acetylated lysine residues. Many transcription and chromatin regulatory proteins contain bromodomains, and may be recruited to gene promoters, in part, through binding of acetylated histone tails. Histone acetylation is mediated by histone acetyltransferases (HATs), such as CBP/p300, GCN5L2, PCAF and Tip60, which are recruited to genes by DNA-bound protein factors to facilitate transcriptional activation. De-acetylation, which is mediated by histone deacetylases (HDAC and Sirtuin proteins), reverses the effects of acetylation and generally facilitates transcriptional repression. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence (IF-IC): 1:100, Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. , Immunoprecipitation: 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: | Anti-Histone H2B type 1-B |
Supplier: | United States Biological |
Supplier-Nr: | H5110-09Z |
Properties
Application: | IF, IP, WB |
Antibody Type: | Polyclonal |
Conjugate: | No |
Host: | Rabbit |
Species reactivity: | human, monkey, mouse, rat |
Immunogen: | Synthetic peptide containing acetylated Lys12 of human histone H2B. |
Format: | Affinity Purified |
Database Information
KEGG ID : | K11252 | Matching products |
UniProt ID : | P33778 | Matching products |
Gene ID | GeneID 3018 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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