Anti-citrullinated-Interleukin-6

Interleukin-6 (IL-6) is a cytokine with roles in both the initiation and resolution of inflammatory responses. IL-6 is produced in response to an inflammatory event, induces recruitment of neutrophils to the site of inflammation, and signals through the membrane-bound IL-6 receptor (IL-6R) to upregulate the synthesis of acute phase proteins, lipolysis in the liver, and induced differentiation of T and B cells. Following initiation of this classical signaling pathway, a soluble form of IL-6R (sIL-6R) is released from neutrophils to promote recruitment of monocytes and macrophages, induction of fever via IL-6 action in the hypothalamus, and production of anti-inflammatory M2 macrophages to initiate tissue repair. Production of IL-6 is dysregulated in various chronic inflammatory diseases, including rheumatoid arthritis, Castleman disease, polymyalgia rheumatica, and giant cell arteritis. It also induces tumor growth and metastasis via activation of JAK/STAT signaling. Recombinant human IL-6 can be citrullinated at arginine residues by protein arginine deiminase 4 (PAD4) in vitro. Citrullinated IL-6 and anti-citrullinated peptide antibodies (ACPAs) reactive with citrullinated IL-6 have been found in synovial fluid and sera, respectively, from patients with rheumatoid arthritis. Cayman's Citrullinated Interleukin-6 Polyclonal Antibody can be used for ELISA and Western blot (WB) applications. The antibody recognizes citrullinated IL-6 at ~24 kDa from human samples.Synonyms: B Cell Stimulatory Factor 2, BSF-2, CDF, CTL Differentiation Factor, HGF, Hybridoma Growth Factor, IFN-beta2, IL-6, Interferon-beta2. Immunogen: Synthetic citrullinated peptide corresponding to an internal region of human IL-6. MW: ~24 kDa. Formulation: (Request formulation change), 1 ml of peptide affinity-purified polyclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Rabbit. Applications: ELISA, WB. Cross Reactivity: (+) Citrullinated IL-6. UniProt Accession #: P05231.