Anti-Vimentin

Human vimentin is a 53,521kD protein (466aa) expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton. In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the PNS, and by neural stem cells of the CNS. In some forms of glioblastoma, vimenin is reexpressed GAP-43 by the tumor cells and serves as a general indicator of their undifferentiated state. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested. , Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues, Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells, Western Blot: 1:2000-1:5000, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch , I1906-10 IgY, Chicken (HRP) Rb x Ch , I1906-12 IgY, Chicken (FITC) Rb x Ch , I1906-14 IgY, Chicken (AP) Rb x Ch, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.