Anti-Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta Chain, TUBB, Tubulin beta-5

Tubulin is a major cytoskeleton component that has five distinct forms. alpha and beta Tubulins form heterodimers which multimerize to form a microtubule filament. There are five beta Tubulin isoforms (b1, b2, b3, b4A and b4B) that are expressed in mammalian tissues. b1 and b4 are present throughout the cytosol, b2 is present in the nuclei and nucleoplasm, and b3 is a neuron-specific cytoskeletal protein. Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100-1:400, Western Blot: 1:1000-1:4000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.