Anti-trimethyl Histone H3 (Lys4) Rabbit (Clone RM137)

Histone H3 undergoes many modifications including acetylation, methylation, and phosphorylation that are important for regulation of gene transcription. Methylation at lysine 4 of histone 3 (H3K4) is an evolutionarily conserved modification associated with transcriptionally active chromatin. The SET1/MLL (KMT2) family of methyltransferases is the principal enzyme family responsible for H3K4 methylations. This antibody reacts to histone H3 trimethylated at lysine 4 (K4me3) and does not detect monomethylated lysine 4 (K4me1), dimethylated lysine 4 (K4me2), or other methylations in histone H3.Immunogen: A trimethyl-peptide corresponding to trimethyl-histone H3 (Lys4). Clone Designation: RM137. Formulation: (Request formulation change), 100 µg of protein A-affinity purified antibody from an animal origin free culture supernatant. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, multiplex-based assays, WB. Cross Reactivity: (+) Histone H3 trimethylated at lysine 4 (K4me3), (-) Monomethylated lysine 4 (K4me1), (-) Dimethylated lysine 4 (K4me2), (-) Other methylations in histone H3.