Anti-TLR3 (CD283) (APC)

Human TLR3 is a 116kD type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. TLR3 is found in phagosomes, where the acidic pH enables binding of internalized double-stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells. Ligand binding by TLR3 induces receptor dimerization, leading to the release of inflammatory cytokines and dendritic cell maturation. TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes, and is upregulated by IFN-beta and LPS.4, 8-10 TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection. The extracellular domain (ECD) of human TLR3 shares 80%, 79%, and 77% aa sequence identity with the ECD of rat, mouse, and bovine TLR3, respectively. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at 12 months at 4°C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyaninconjugates are sensitive to light.