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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
T5048-04.100 | 100 µg | - | - |
3 - 19 business days* |
916.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Thrombomodulin is a 110-120kD, endothelial cell-specific type I transmembrane glycoprotein. It... more
Product information "Anti-Thrombomodulin"
Thrombomodulin is a 110-120kD, endothelial cell-specific type I transmembrane glycoprotein. It binds thrombin, rendering it unavailable for clotting, and activates protein C, which also serves as an anticoagulant. Mouse Thrombomodulin is 577aa in length. It contains a 510aa extracellular domain (ECD) (aa17-517) that shows one C-type lectin domain (aa31-167) and six EGF-like regions (aa240-480). Thrombin binds to aa403-480. One isoform may exist that shows a deletion of the C-terminal 86 amino acids (aa492-577). Over aa17-517, mouse Thrombomodulin is 66%, 85% and 67% aa identical to human, rat and canine TM, respectively. Applications: Suitable for use in Flow Cytometry, Direct ELISA, Western Blot Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/ml, 10ul labels 1x10e6 bEnd. cells , Western Blot: 1ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Thrombomodulin is ~25ng/lane under non-reducing conditions. Use under reducing conditions is not recommended., Immunohistochemistry: 8-25ug/ml detects Thrombomodulin in frozen sections of normal mouse kidney and heart., Immunocytochemistry: 8-25ug/ml. Used with bEnd. cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. Between each step, cells were washed with PBS, BSA., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | Anti-THBD, Anti-THRM |
Supplier: | United States Biological |
Supplier-Nr: | T5048-04 |
Properties
Application: | ELISA, FC, ICC, IHC, WB |
Antibody Type: | Monoclonal |
Clone: | 8H203 |
Conjugate: | No |
Host: | Rat |
Species reactivity: | mouse |
Immunogen: | NS0-derived rmThrombomodulin. (aa 17 - 517) |
Format: | Purified |
Database Information
KEGG ID : | K03907 | Matching products |
UniProt ID : | P07204 | Matching products |
Gene ID : | GeneID 7056 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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