Anti-SOAT-2/ACAT-2

Sterol O-acyltransferase 2 (SOAT-2), also known as acyl-coenzyme A:cholesterol acyltransferase-2 (ACAT-2), is an enzyme encoded by ACAT2 in humans that catalyzes the intracellular formation of cholesterol esters from cholesterol and long-chain fatty acyl-coenzyme A. It is constitutively expressed in the liver and small intestine and localized to the rough endoplasmic reticulum where it preferentially utilizes oleic acid (Cay-90260, Cay-24659) or palmitic acid (Cay-10006627) as fatty acid substrates for the synthesis of cholesterol esters, which are stored intracellularly or packaged into chylomicrons or VLDL and secreted into the blood stream. SOAT-2/ACAT-2 protein levels are increased in macrophages under various pathological conditions, including atherosclerosis. SOAT-2/ACAT-2 activity and protein levels are increased by cholesterol in vitro and in vivo, and ACAT2 expression is increased by the transcription factors hepatocyte nuclear factor 1-alpha (HNF-1-alpha) and HNF-4-alpha in Caco-2 cells and human liver, respectively. Genome-wide deletion of Acat2 reduces intestinal cholesterol absorption and decreases hepatic, but not plasma, total cholesterol levels in mice fed a high-cholesterol diet. Liver ACAT2 activity is negatively correlated with plasma HDL-C and ApoA1 in normolipidemic patients. Cayman's SOAT-2/ACAT-2 Polyclonal Antibody can be used for immunofluorescence (IF), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes the N-terminal region of SOAT-2/ACAT-2 at approximately 60 kDa from human, mouse, ovine, porcine, and rat samples.Synonyms: Acyl-coenzyme A:Cholesterol Acyltransferase-2, Cholesterol Acyltransferase 2, Sterol O-Acyltransferase 2. Immunogen: Synthetic peptide from the N-terminal region of human SOAT-2/ACAT-2. Formulation: (Request formulation change), 500 µl of peptide affinity-purified polyclonal antibody.. Storage Buffer: TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide. Host: Rabbit. Applications: IF, IHC, WB. UniProt Accession #: O75908. Origin: Animal/Rabbit.