Anti-Postsynaptic Density Protein 95 (PSD-95, PSD95, Discs Large Homolog 4, DLG4, Human Post-synapti

PSD-95 belongs to a class of proteins referred to as MAGUKs (membrane associated guanylate kinases). Cannonical MAGUK proteins contain one or three PDZ (PSD-95/discs large/zona occludens-1) domains, an SH3 domain, and a guanylate kinase homology domain. PSD-95 and several other MAGUK and PDZ containing proteins (eg. PSD-93, SAP-97, SAP-102, MALS/Veli proteins(2)) are enriched in the post synaptic density.(3) These proteins appear to regulate synaptic function by acting as molecular scaffolds to organize and cluster signaling machinery at synapses, in part by interaction of their PDZ domains with carboxy-terminal T/SXV motifs present on synaptic proteins including NMDA receptors and Shaker type K+ channels. PSD-95 has also been shown to link NMDA receptors to the downstream signal transduction protein neuronal nitric oxide synthetase (nNOS) via a novel PDZ:PDZ interaction with nNOS. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:8000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.