Anti-Ornithine Decarboxylase (ODC)

Ornithine decarboxylase is the rate-limiting enzyme in polyamine biosynthesis converting ornithine into putrescine. In normal tissue ornithine decarboxylase activity is low but increases in proliferating tissue. Applications: Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunofluorescence: 1:500-1:800 with overnight incubation at 4°C., Immunohistochemistry: Frozen tissue sections and on paraffin embedded material when using the microwave retrieval method., Optimal dilutions to be determined by the researcher. Positive Control: Stefanini-fixed frozen sections of renal cortex from testosterone-treated mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.