Anti-Nicotinic Acetylcholine Receptor alpha 1 (nAChR, Acetylcholine Receptor Protein alpha Chain Pre

The acetylcholine receptor of muscle, like the nicotinic acetylcholine receptor of the Torpedo electric organ, has 5 subunits of 4 different types: 2 alpha and 1 each of beta, gamma, and delta subunits. The alpha subunit exists in 2 isoforms. The protein-coding sequence of the human ACHRA gene is divided into 9 exons that correspond to different structural and functional domains of the precursor molecule. Human nicotinic acetylcholine receptor genes alpha is assigned to chromosome 2. Mutation of the acetylcholine receptor alpha subunit causes a slow-channel myasthenic syndrome by enhancing agonist binding affinity. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml , Immunohistochemistry (paraffin): 1-2ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.