Anti-NFkB p65, acetaldehyde (Lys310) (NF-kB p65, NF-kB, Transcription Factors of the Nuclear Factor

Transcription factors of the nuclear factor k B (NF-kB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-kB1 (p105/p50), and NF-kB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-kB is sequestered in the cytoplasm by IkB inhibitory proteins (3-5). NF-kB-activating agents can induce the phosphorylation of IkB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-kB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKa (IKK1) regulate the phosphorylation and processing of NF-kB2 (p100) to produce p52, which translocates to the nucleus (9-11). NF-kB assembly with IkB, DNA binding, and transcriptional activity, are regulated by p300/CBP acetytransferases that principally target Lys218, Lys221, and Lys310 (12-14). This process is reciprocally regulated by histone deacetylases (HDACs), several HDAC inhibitors have been shown to activate NF-kB (12-14). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunoprecipitation: 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.