Anti-MxI1 (MAX Interactor 1, MAX-interacting Protein 1)

Mxi-1 (Max interactor 1) is a 26kD member of the bHLH-leucine zipper family, Myc superfamily of transcription factors. It is a widely expressed transcriptional repressor that heterodimerizes with Max and blocks Max-Myc gene activation. Human Mxi-1 is 228 amino acids (aa) in length. It contains a DNA binding motif (aa 69-80) and a bHLH domain (aa 81-120). Multiple splice variants of Mxi-1 appear to exist. They may incorporate an alternate start site at Met37, a 92 aa substitution for the first 26 N-terminal amino acids, or a deletion of the C-terminal 59 or 74 amino acids. Over aa 81-228, human Mxi-1 is 95% aa identical to mouse Mxi-1. Applications: Suitable for use in Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilution: Immunocytochemistry: 10ug/ml, ELISA: 0.5-1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.