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![Anti-Mast Cell Chymase (CMA1)](/custom/plugins/NetiThemeBiomol/Resources/Themes/Frontend/Biomol/frontend/_public/src/img/no-picture.jpg)
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
M2414-01D.100 | 100 µg | - | - |
3 - 19 business days* |
916.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Chymase-1 (also mast cell protease I) is a secreted, 30kD glycoprotein member of the granzyme... more
Product information "Anti-Mast Cell Chymase (CMA1)"
Chymase-1 (also mast cell protease I) is a secreted, 30kD glycoprotein member of the granzyme subfamily, peptidase S1 family of enzymes. It is secreted by mast cells and vascular smooth muscle cells, and converts angiotensin I into angiotensin II. This promotes fibroblast proliferation and secretion of ECM. Mature human chymase is 226aa in length. It contains one peptidase S1 domain (aa 2-245). There is one potential alternate splice form. It shows a deletion of the 99 C-terminal amino acids (aa149-247). Applications: Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Direct ELISA: 0.5-1ug/ml. Detection limit for rhChymase is ~1ng/well., Western Blot: 1-2ug/ml. Detection limit for rhChymase is , ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection wil increase sensitivity by 5 to 50 fold., Immunoprecipitation: 25ug/ml from conditioned cell culture medium., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | Anti-CYH, EC=3.4.21.39, Anti-Alpha-chymase, Anti-Mast cell protease I |
Supplier: | United States Biological |
Supplier-Nr: | M2414-01D |
Properties
Application: | ELISA, IP, WB |
Antibody Type: | Monoclonal |
Clone: | 8H183 |
Conjugate: | No |
Host: | Mouse |
Species reactivity: | human |
Immunogen: | NS0-derived rhChymase. (aa 20 - 247) |
Format: | Purified |
Database Information
KEGG ID : | K01329 | Matching products |
UniProt ID : | P23946 | Matching products |
Gene ID | GeneID 1215 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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