Anti-IRF3 (Clone 1E10)

Interferon regulatory factor 3 (IRF3) is a member of the IRF family that plays a crucial role in activation of innate immunity and inflammation in response to viral infection, functioning as a molecular switch for antiviral activity. Double-stranded RNA generated during a viral infection leads to IRF3 activation through serine/threonine phosphorylation by TBK1 (Cay-22817) or IKKepsilon (IKBKE) kinases, which induces a conformational change leading to its dimerization, nuclear localization, and association with CREB binding protein (CREBBP)/p300. The complex formed by this association, known as DRAF1, activates transcription of interferon alpha (IFN-alpha) and IFN-beta as well as other IFN-induced genes, which play a critical role in the type 1 IFN-dependent immune response. Cayman's IRF3 Monoclonal Antibody can be used for Western blot and ELISA applications. The antibody recognizes IRF3 at ~47 kDa from human samples.Synonyms: Interferon Regulatory Factor 3. Immunogen: IRF3 (human recombinant). Clone Designation: 1E10. Formulation: (Request formulation change), 100 µg of protein G-purified antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Mouse. Isotype: IgG2a. Applications: ELISA and WB. UniProt Accession #: Q14653.