Anti-Histone H4

Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells. Applications: Suitable for the detection of Histone H4 by Western blot. Human peripheral blood cell lysate or Jurkat cell lysate can be used as positive control. An approximately 14kD band is detected. Western blot analysis of Histone H4 in human PBMC cell lysate with antibody. Recommended Dilution: Western blot: 2ug/ml, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.