Anti-Histone H3K4Me2

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Dimethylation of H3K4 is catalyzed by SET domain-containing histone methyltransferases, including SET1, MLL1, -2, -3, and -4, and SMYD3, and dysregulation of these methyltransferases is associated with various diseases, including breast and prostate cancer and Alzheimer's disease in humans. H3K4Me2 is highly enriched in the promoter region of transcriptionally active genes, as well as primed, inactive genes that are targeted for future transcription. Cayman's Histone H3K4Me2 Polyclonal Antibody can be used for ELISA and Western blot applications. It detects H3K4Me2 at ~16 kDa from human samples.Synonyms: Histone H3 containing dimethylated lysine 4. Immunogen: Synthetic peptide from the N-terminal region of human Histone H3 (dimethyl K4) conjugated to KLH. Formulation: (Request formulation change), 500 µl of peptide affinity-purified polyclonal antibody. Storage Buffer: TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide. Host: Rabbit. Applications: ELISA and WB. Cross Reactivity: (+) Histone H3 monomethylated at K4 (~100%), (+) Histone H3 trimethylated at K4 (~20%), (+) Histone H3 dimethylated at K9 (~20%). UniProt Accession #: P68431.