Anti-HIF1A (HyP564) (HIF1A, BHLHE78, MOP1, PASD8, Hypoxia-inducible factor 1-alpha, ARNT-interacting

HIF1A is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, FLISA, Recommended Dilution: FLISA: 1:1,000, Western Blot: 1:100-500, Flow Cytometry: 1:10-50, Immunofluorescence: 1:10-50, Immunohistochemistry: 1:10-50, Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. , , Note: Applications are based on unconjugated antibody.