Anti-Golgi Protein, 58K, NT (FTCD, LCHC1, formiminotransferase cyclodeaminase, formimidoyltransferas

A58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency, also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. pplications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Peptide ELISA: >1:64,000 , Western Blot: 0.03-0.5ug/ml. A 55-60kD band observed in Human Liver lysate (calculated MW of 58.9kD according to NP_006648), Immunohistochemistry: 3-5µg/ml In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. , Optimal dilutions to be determined by the researcher. Domain Information: COG3404: Methenyl tetrahydrofolate cyclohydrolase [Amino acid transport and metabolism], COG3643: Glutamate formiminotransferase [Amino acid transport and metabolism] , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See G8577-50B-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.